Localization of the calreticulin gene mRNA in unpollinated and pollinated styles of Petunia hybrida

• Authors: M. Lenartowska , R. Lenartowski , E. Bednarska
• Languages of publication: EN

Abstracts

EN

The objective of our research on Petunia hybrida is to understand the role of calreticulin in the growth of pollen tubes in the pistil. The aim of this study was the first step: finding out whether CRT gene expression takes place in unpollinated and pollinated styles. It was revealed by in situ hybridization that the transcription of the calreticulin gene takes place in the transmitting cells of unpollinated and pollinated styles and in pollen tubes growing in vivo. The mRNA transcripts of the CRT gene were localized mainly on the surface of endoplasmic reticulum (ER) membranes, both in transmitting cells and in the tip cytoplasm of pollen tubes. The results of this study show that calreticulin can be involved in pollen ? pistil interaction in vivo.

Keywords

EN

CRT GENE; PETUNIA HYBRIDA; POLLEN TUBE; TRANSCRIPTION; TRANSMITTING CELL

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2001
• Volume: 42
• Issue: 1
• Pages: 15-20

Contributors

author

M. Lenartowska

author

R. Lenartowski

author

E. Bednarska

• Document Type: ARTICLE
• Publication order reference: M. Lenartowska, Laboratory of Developmental Biology, Institute of General and Molecular Biology, N. Copernicus University, Gagarina 9, 87-100 Torun, Poland, e-mail: [e-mail]