Characterization of beta-adrenergic receptors in duck cerebral cortex

• Authors: H.J.B. Zawilska , J. Zalewska-Kaszubska , G. Marczak , D. Gorska , A. Woldan-Tambor
• Languages of publication: EN

Abstracts

EN

Beta-adrenoceptor binding sites were characterized in duck cerebral cortex by an in vitro binding technique, using [3H]dihydroalprenolol ([3H]DHA) as a receptor-specific radioligand. The specific binding of [3H]DHA to duck cerebral cortical membranes was found to be rapid, stable, saturable, reversible, and of high affinity. Saturation analysis resulted in a linear Scatchard plot suggesting binding to a single class of receptor binding sites with high affinity (Kd = 1.18 nM) and high capacity (Bmax = 162 fmol/mg protein). Competition studies showed the following relative rank order of potency of various compounds to inhibit the [3H]DHA binding: antagonists - ICI 118,551 > S(-)-propranolol >> betaxolol, yohimbine, WB-4101, prazosin, mianserine; agonists - isoprenaline @ fenoterol > salbutamol >> clonidine, phenylephrine. The obtained data suggest that in duck cerebral cortex beta-adrenergic receptors (like those described in brains of chick and pigeon) are of the beta2 subtype. This is in contrast to what has been reported for the mammalian brain, where - among b-adrenoceptors - the beta1 subtype is predominant.

Keywords

EN

ADRENERGIC RECEPTOR; CEREBRAL CORTEX; DUCK

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2000
• Volume: 60
• Issue: 3
• Pages: 301-307

Contributors

author

H.J.B. Zawilska

author

J. Zalewska-Kaszubska

author

G. Marczak

author

D. Gorska

author

A. Woldan-Tambor

• Document Type: article
• Publication order reference: J.B. Zawilska, Department of Pharmacodynamics, Medical University of Lodz, 1 Muszynski St., 90-151 Lodz, Poland