Sphingolipid derivatives modulate intracellular Ca2+ in rat synaptosomes

• Authors: B.G. Miguel , M.C. Calcerrada , R.E. Catalan , A.M. Martinez
• Languages of publication: EN

Abstracts

EN

Sphingosylphosphorylcholine (SPC) induces a rapid increase of intracellular Ca2+ concentration in isolated synaptosomes. This effect is dose-dependent and is also dependent on extracellular Ca2+. Sphingosine (SPH) has a smaller effect and treatment with psychosine (PSY) is ineffective, which suggests that phosphorylation of the 1-carbon of SPH is required for the SPC to act as a Ca2+ release agonist in synaptosomes. Experiments performed in the presence of heparin or ryanodine indicate that SPC-elicited Ca2+ release is not mediated by IP3 or ryanodine receptors. Finally, our results show that the effect of SPC on Ca2+ concentration is nimodipine-sensitive, suggesting that SPC possibly activates a specific sphingolipid-gated Ca2+ channel in synaptosomes.

Keywords

EN

CALCIUM; CALCIUM CHANNEL; RAT; SPHINGOLIPID; SYNAPTOSOME

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2001
• Volume: 61
• Issue: 2
• Pages: 113-117

Contributors

author

B.G. Miguel

author

M.C. Calcerrada

author

R.E. Catalan

author

A.M. Martinez

• Document Type: ARTICLE
• Publication order reference: B. G. Miguel, Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, E-28040 Madrid, Spain