The influence of exogenous growth regulators on germination and conversion in somatic embryos of Norway spruce (Picea abies (L.) Karst.)

• Authors: M.J. Latkowska , K. Wojciechowska
• Languages of publication: PL

Abstracts

EN

Modification of the medium composition was a trial to improve germination and conversion in somatic embryos of Norway spruce. The mature embryos germinated for 1 week in the medium supplemented with either auxin (IAA, IBA, NAA ? 0.05; 0.25; 0.1 or 0.5 muM) or gibberellin (GA3, GA4+7 - 25; 50; 125 or 250 mg dm-3). The hormone-free medium used for the next 2 weeks of germination was enriched or not with activated charcoal (10 mg dm-3). The auxin applied in the medium did not affect embryo germination nor root and shoot growth. GA3 stimulated root and shoot growth in germinating somatic embryos, whereas the highest concentration of GA4+7 completely inhibited embryo germination.

Keywords

EN

AUXIN; DRYING; GIBBERELLIN; SOMATIC EMBRYOGENESIS

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 107-113

Contributors

author

M.J. Latkowska

author

K. Wojciechowska

• Document Type: ARTICLE
• Publication order reference: M.J. Latkowska, Katedra Roslin Ozdobnych, Szkola Glowna Gospodarstwa Wiejskiego, ul. Nowoursynowska 166, 02-787 Warszawa, Poland