Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana

• Authors: G. Perrella , G. Cremona , F. Consiglio , A. Errico , R.A. Bressan , C. Conicella
• Languages of publication: EN

Abstracts

EN

In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.

Keywords

EN

ACTIVATION TAGGING; ARABIDOPSIS THALIANA; GAMETOPHYTIC MUTATION; SEXUAL REPRODUCTION; SPOROPHYTIC MUTATION; T-DNA

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2006
• Volume: 47
• Issue: 2
• Pages: 109-111

Contributors

author

G. Perrella

author

G. Cremona

author

F. Consiglio

author

A. Errico

author

R.A. Bressan

author

C. Conicella

• Document Type: ARTICLE
• Publication order reference: C. Conicella, CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita 133, 80055 Portici, Italy