Effect of K+ accumulation removal and high extracellular osmolarity on K+ current in insect axonal membrane

• Authors: M. Stankiewicz , M. Pelhate , I. Pancerzynska , A. Zienkiewicz , Kadziela^W. , W. Kadziela
• Languages of publication: EN

Abstracts

EN

The effect of removal of K+ accmulation on K+ current in insect axonal membrane was observed. Experiments were performed on isolated giant axon of a cockroach using double oil gap technique. K+ accumulation was reduced by: (1) an outward water flow induced by non-electorlytes (urea, glucose) added to extracellular saline and (2) by an increase of non-specific permeability of axonal glial layer obtained after the application of DMSO. The conclusions are: (1) osmolar effect depends on the type of molecule used for osmotic shock, (2) increase of outward K+ current in conditions of high extraaxonal osmotic pressure is attributed to the decrease of K+ accumulation and outward water flow, (3) removal of K+ accumulation doesn't affect the kinetics and the time course of K+ current, (4) experiments confirmed the presence of an inactivating component in the axonal outward K+ current, (5) DMSO must be used cautiously as a solvent in electophysiological experiments.

Keywords

EN

AXONAL MEMBRANE; OSMOTIC SHOCK; POTASSIUM

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 1996
• Volume: 56
• Issue: 1
• Pages: 95-102

Contributors

author

M. Stankiewicz

author

M. Pelhate

author

I. Pancerzynska

author

A. Zienkiewicz

author

Kadziela^W.

author

W. Kadziela

• Document Type: proceeding
• Publication order reference: M.Stankiewicz