Effect of palmitoylcarnitine on the cellular differentiation, proliferation and protein kinase C activity in neuroblastoma nb-2a

• Authors: K.A. Nalecz , J.E. Mroczkowska , U. Berent , M.J. Nalecz
• Languages of publication: EN

Abstracts

EN

Palmitoylcarnitine is synthesized through the action of palmitoylcarnitine transferase I - an enzyme specifically inhibited by etomoxir. An increase of the intracellular content of palmitoylcarnitine in neuroblastoma NB-2a cells after administration of carnitine was correlated with an inhibition of cell proliferation and a concomitant promotion of differentiation processes. The activity of protein kinase C was measured in vivo, with cells permeabilized through the use of streptolysin O and a peptide substrate. Palmitoylcarnitine inhibited the phorbol ester stimulated reaction of the peptide phosphorylation in a concentration dependent way. The degree of protein kinase C inhibition was correlated with intracellular increase of the palmitoylcarnitine content, pointing to this compound as a natural modulator of protein kinase C activity.

Keywords

EN

DIFFERENTIATION; ETOMOXIR; NEUROBLASTOMA; PALMITOYLCARNITINE; PROLIFERATION; PROTEIN KINASE C

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 1997
• Volume: 57
• Issue: 4
• Pages: 263-274

Contributors

author

K.A. Nalecz

author

J.E. Mroczkowska

author

U. Berent

author

M.J. Nalecz

• Document Type: article
• Publication order reference: K.A. Nalecz, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland, Email: [e-mail].p