Optimization of transduction of chosen cell lines using adenoviral vetors of the first generation

• Authors: M. Stopa , A. Dulak , A. Jozkowicz
• Languages of publication: PL

Abstracts

EN

Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene beta-galactosidase (Adbeta-gal). We optimised the adsorption time and dose of Ad beta-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 IU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adbeta-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.

Keywords

EN

ADENOVIRAL VECTOR; GENE THERAPY; REPORTER GENE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2007
• Issue: 3
• Pages: 123-140

Contributors

author

M. Stopa

author

A. Dulak

author

A. Jozkowicz

• Document Type: ARTICLE
• Publication order reference: Alicja Jozkowicz, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland