Flow cytometry in mammalian sperm sexing and quality measurement

• Authors: M. Bochenek
• Languages of publication: PL

Abstracts

EN

Flow cytometry is a powerful technique for measurements of many structural and functional cell parameters. In sperm analyses it offers several advantages over the formerly used methods: high accuracy, possibility of measuring two or even three parameters simultaneously and analysis of a high number of cells per second. Moreover, at present it is the only technique which allows sperm sorting of X- and Y-fractions with high purity. The paper is a review of the flow cytometry applications in sperm sexing and measurements of quality parameters: membrane integrity, acrosome status, mitochondrial and esterase activity and sperm chromatin structure.

Keywords

EN

FLOW CYTOMETRY; SPERM

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1998
• Issue: 2
• Pages: 119-129

Contributors

author

M. Bochenek

• Publication order reference: M. Bochenek, Zaklad Fizjologii Rozrodu Zwierzat, Instytut Zootechniki, 32-083 Balice k. Krakowa, Poland