alpha-(1->3)-Glucans from cell wall of Laetiporus sulphureus (Bull.:Fr.) Murrill ? isolation, characteristics and application for induction of mutanase synthesis

• Authors: A. Wiater , M. Pleszczynska , J. Szczodrak , K. Prochniak
• Languages of publication: PL

Abstracts

EN

Five different methods described in the literature were used for the isolation of alpha-(1->3)-glucans from the cell wall of fruiting bodies of Laetiporus sulphureus (Bull.:Fr.) Murrill, and their comparative analysis was performed. The separated fungal biopolymers were well-characterized in respect of their structure and some physicochemical properties. Structural analyses, i.e., Fourier-transform infra-red (FT-IR) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy and specific rotation, revealed that the alkali-soluble wall fraction from this basidiomycetous fungus contained about 56% of (1->3)-linked alpha-glucans. Four out of five alpha-(1->3)-glucans isolated by different methods from the mycelium of the polypore fungus L. sulphureus induced higher activity of fungal and bacterial mutanase than those obtained on mutan. Therefore, the alpha-(1->3)-glucans from fruiting bodies of L. sulphureus can be used as a new alternative to streptococcal mutan, which so far has been known as the best inducer of mutanase production

Keywords

EN

GLUCAN; LAETIPORUS SULPHUREUS; MUTANASE; STRUCTURAL ANALYSE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2008
• Issue: 2
• Pages: 174-189

Contributors

author

A. Wiater

author

M. Pleszczynska

author

J. Szczodrak

author

K. Prochniak

• Document Type: ARTICLE
• Publication order reference: Adrian Wiater, Zak?ad Mikrobiologii Przemys?owej, Instytut Mikrobiologii i Biotechnologii, Uniwersytet Marii Curie-Sk?odowskiej, ul. Akademicka 19, 20-033 Lublin, Poland