Analysis of physiological state of single bacterial cell using fluorescent staining methods

• Authors: J. Sadowska , W. Grajek
• Languages of publication: PL

Abstracts

EN

The paper reviews fluorescent staining techniques allowing to diagnose the physiological state of bacterial cells. Different staining probes and a manner of their use for analysis of cell viability, membrane potential, membrane integrity, intracellular pH, respiratory activity, amount of nucleic acids, and activity of chosen intracellular enzymes are described. Range of examples of fluorescent staining for monitoring of physiological state of bacteria in natural environment and in biotechnological processes are presented.

Keywords

EN

BACTERIA; DEHYDROGENASE; DNA; ESTERASE; FLOW CYTOMETRY; FLUORESCEIN; FLUORESCENT STAINING

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2009
• Issue: 4
• Pages: 102-114

Contributors

author

J. Sadowska

author

W. Grajek

• Document Type: ARTICLE
• Publication order reference: Justyna Sadowska, Katedra Biotechnologii i Mikrobiologii Zywnosci, Uniwersytet Przyrodniczy, ul. Wojska Polskiego 48, Poznan, Poland