Effects of lead on cholinergic SN56 neuroblastoma cells

• Authors: A. Jankowska-Kulawy , S. Gul-Hinc , H. Bielarczyk , J.B. Suszkiw , T. Pawelczyk , A. Dys , A. Szutowicz
• Languages of publication: EN

Abstracts

EN

Age-dependent accumulation of lead in brain has been implicated in the pathomechanisms of Alzheimer.s disease. The aim of this work was to investigate whether cholinotoxic effects of lead may result from alterations in acetyl-CoA metabolism. One day exposure of differentiated SN56 cholinergic neuroblastoma cells to 0.5 mumol/L lead or 0.01 mmol/L amyloid-beta1-42 increased fraction of nonviable cells to about 20%. Suppression of choline acetyltransferase activity occurred only in the presence of fresh amyloid-beta1-42, whereas lead was ineffective. All agents in combination caused suppression of acetyl-CoA in cytoplasm and mitochondria down to 19% and 34% of controls, respectively. Inverse correlation was observed between whole cell acetyl-CoA level and fraction of nonviable cells at different combinations of lead and other neurotoxic compounds. It indicates that lead had no primary suppressive effect on cholinergic phenotype but, at least in part, exerted cytotoxic influence on cholinergic neurons through the decrease of their acetyl-CoA.

Keywords

EN

ACETYL-COA; AMYLOID BETA; CHOLINE ACETYLTRANSFERASE; LEAD; NEUROTOXICITY

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2008
• Volume: 68
• Issue: 4
• Pages: 453-462

Contributors

author

A. Jankowska-Kulawy

author

S. Gul-Hinc

author

H. Bielarczyk

author

J.B. Suszkiw

author

T. Pawelczyk

author

A. Dys

author

A. Szutowicz

• Document Type: ARTICLE
• Publication order reference: Andrzej Szutowicz, Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland