Sphingosylphosphorylcholine induces mitochondria-mediated apoptosis in neuro 2a cells: Involvement of protein kinase C

• Authors: B.G. Miguel , I. Fernandez , I. Toboso , A. Agudo-Lopez , E. Catalan , A.M. Martinez
• Languages of publication: EN

Abstracts

EN

We demonstrate that sphingosylphosphorylcholine-mediated cell death involves the activation of different protein kinase C isozymes in different manners. Treating cells with sphingosylphosphorylcholine resulted in activation of protein kinase C delta, which is necessary, together with elevation of Ca2+, for sphingosylphosphorylcholine-induced apoptosis. A rapid translocation from cytosol to membrane, and a proteolytic protein kinase C delta cleavage was found, probably due to activation of caspase-3, to give a catalytically active fragment involved in cellular apoptosis. Moreover, sphingosylphosphorylcholine also induced translocation of protein kinase C zeta, resulting in an anti-apoptotic effect. To explore whether a mitochondrial pathway is involved in sphingosylphosphorylcholine-induced apoptosis, we analyzed the effect of sphingosylphosphorylcholine on cytochrome c release and caspase-3 activity. We must point out that the sphingolipid caused an increase of cytochrome c release from mitochondria to cytosol concomitantly with an increase of caspase-3 activity. Furthermore, a translocation of Bax was found, after sphingosylphosphorylcholine treatment.

Keywords

EN

APOPTOSIS; NEUROBLASTOMA; PROTEIN KINASE C; SPHINGOSYLPHOSPHORYLCHOLINE

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2008
• Volume: 68
• Issue: 4
• Pages: 443-452

Contributors

author

B.G. Miguel

author

I. Fernandez

author

I. Toboso

author

A. Agudo-Lopez

author

E. Catalan

author

A.M. Martinez

• Document Type: REVIEW
• Publication order reference: Ana M. Martinez, Departamento de Bioquimica y Biologia Molecular I, Facultad de Quimica, Universidad Complutense de Madrid, 28040 Madrid, Spain