New effective method of obtaining chlorate-resistant cell lines of monohaploid potato

• Authors: B. Chwilkowska
• Languages of publication: EN

Abstracts

EN

The studies were performed with the aim to develop a more effective method for obtaining in vitro chlorate-resistant potato cell lines with genetic changes of enzymes participating on mineral assimilation and used for studying metabolism of this pathway.These lines were isolated from Solanum tuberosum monohaploid cells (2n=x=12), plated into selection media, differing in KClO3 concentration and incubated in the suspension.Duplication of cell population in the suspension occured at day 7 of culture.A results of performed cell selection were 13 chlorate resistant cell lines, which may be referred to the so-called "stable variants".

Keywords

EN

CELL SURVIVAL; CHLORATE-RESISTANCE; MONOPLOID POTATO; MUTAGENESIS EFFICIENCY; PLATING EFFICIENCY; RESISTANT VARIANT

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Genetica Polonica
• Year: 1994
• Volume: 35
• Issue: 4
• Pages: 303-314

Contributors

author

B. Chwilkowska

• Document Type: article
• Publication order reference: B.Chwilkowska