SSCP polymorphism within 5' region of bovine lactoglobulin (LGB) gene

• Authors: S. Kaminski , T. Zabolewicz

Abstracts

EN

In the paper the detection of the SSCP polymorphism within the 5' fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5' fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named R1, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.

Keywords

EN

BETA-LACTOGLOBULIN; CATTLE; POLYMORPHISM

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1998
• Volume: 39
• Issue: 1
• Pages: 97-102

Contributors

author

S. Kaminski

author

T. Zabolewicz

• Publication order reference: S. Kaminski, Department of Animal Genetics, University of Agriculture and Technology, M. Oczapowskiego 5, 10-719 Olsztyn, Poland