Crystalization of nucleic acids

• Authors: D.A. Adamiak
• Languages of publication: PL

Abstracts

EN

Crystalization is the most important and chellenging step in the determination of the structure of biomolecules and macromolecules by diffracton methods.Macromolecules are extremely complex physical-chemical system whose properties vary as a function of many environmental influences such as temperature, pH, ionic strength and solvent composition.Therefore, a number of factors influencing macromolecule crystal growth ought to be considered.Despite of consderable interest in the approaches allowing for continuous monitoring and setting of crystal growth conditions, in practice, numerous trials ought to be performed in order to obtain appropriate crystals characterized by their optimal geometrical parameters,crystallographic symmetry, unit cell and, most importantly,their high resolution.The vapour diffusion and the microdialysis methods are discussed as the most widely used crystallization methods of macromolecules.

Keywords

EN

CRYSTALLIZATION; NUCLEIC ACID

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1993
• Issue: 4
• Pages: 137-146

Contributors

author

D.A. Adamiak

• Document Type: article
• Publication order reference: D.A.Adamiak