CD80 and CD86 expression on LPS-stimulated monocytes and effect of CD80 and CD86 blockade on IL-4 and IFN-gamma production in nonatopic bronchial asthma

• Authors: R. Rutkowski , T. Moniuszko , A. Stasiak-Barmuta , B. Kosztyla-Hojna , M. Alifier , K. Rutkowski , A. Tatarczuk-Krawiel
• Languages of publication: EN

Abstracts

EN

CD80 and CD86 seem to play an important role in the allergen induced secretion of IL-5 and IL-13. Up till now, the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes and kinetics of these molecules expression on lipopolysaccharide?stimulated monocytes in nonatopic asthma have not been defined. Using monoclonal antibodies we have compared the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes of healthy persons and nonatopic asthmatic patients. We have also assessed the effect of CD80 and CD86 inactivation on interleukin (IL)-4 and interferon gamma (IFN-gamma production in nonatopic asthmatics and healthy subjects. We found that low expression of CD80 on studied monocytes (1.64+0.65 vs. 3.53+1.43%) and moderate expression of CD86 (41.25+134 vs. 49.46+11.49%) were characteristic for asthma. In nonatopic asthma patients inactivation of CD80 or CD86 blockade significantly reduced IFN-gamma production by T lymphocytes (p<0.02; p<0.03). In both studied groups anti-CD80 antibodies did not diminish T lymphocytes` production of IL-4. However anti-CD86 antibodies significantly (p<0.04) reduced the IL-4 concentration in culture supernatants. Our results confirm that both CD80 and CD86 molecules play on important role in the maintenance and amplification of inflammatory process. It suggests that in the inflammatory process that occurs in the nonatopic bronchial asthma Th1 as well as Th2 lymphocytes are equally important

Keywords

EN

BRONCHIAL ASTHMA; INTERFERON GAMMA; INTERLEUKIN; LYMPHOCYTE; MONOCYTE

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2003
• Volume: 51
• Issue: 6
• Pages: 421-428

Contributors

author

R. Rutkowski

author

T. Moniuszko

author

A. Stasiak-Barmuta

author

B. Kosztyla-Hojna

author

M. Alifier

author

K. Rutkowski

author

A. Tatarczuk-Krawiel

• Document Type: ARTICLE
• Publication order reference: R. Rutkowski, Department of Pediatric Allergology, Medical University, Bialystok, Poland