Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig

• Authors: K. Svobodova , K. Bilek , A. Knoll
• Languages of publication: EN

Abstracts

EN

The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.

Keywords

EN

GENE STABILITY; PCR; SUS SCROFA

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2008
• Volume: 49
• Issue: 3
• Pages: 263-265

Contributors

author

K. Svobodova

author

K. Bilek

author

A. Knoll

• Document Type: ARTICLE
• Publication order reference: K. Svobodova, Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry, Brno, Czech Republic