Experimental crosses with and without risk of sperm competition were performed in the locust Locusta migratoria, an orthopteran species with a certain degree of first male sperm precedence. The results showed that first mating males perform a significantly higher number of matings than non-first ones, which is paralleled to a shorter remating period. Possible explanations of this differential male mating frequency are discussed in the light of current hypotheses on sexual selection theory.