Identification of gadoid species (Pisces, Gadidae) by PCR-RFLP analysis

• Authors: F. Aranishi , T. Okimoto , S. Izumi
• Languages of publication: EN

Abstracts

EN

A rapid PCR-RFLP analysis was optimized to identify the presence of 3 closely related gadoid fish species: Alaska pollack Theragra chalcogramma, Pacific cod Gadus macrocephalus and Atlantic cod Gadus morhua in commercial seafood products. Gadoid universal primers were designed for PCR amplification of a 558-bp fragment encoding the mitochondrial cytochrome b gene. Without purification of the PCR products, double digestion with Eco32I and Eco105I restriction enzymes generated reproducible species-specific restriction patterns visualizing 3 fragments (106 bp, 161 bp and 291 bp) in Alaska pollack and 2 fragments (106 bp and 452 bp) in Pacific cod, whereas no cleavage was observed in Atlantic cod. This PCR-RFLP analysis is simple, rapid and reliable, and therefore can be routinely applied to discover fraudulent substitution among 3 economically important gadoid species in commercial seafood products.

Keywords

EN

CYTOCHROME B; GADOID FISH; PCR-RFLP; SPECIES IDENTIFICATION

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2005
• Volume: 46
• Issue: 1
• Pages: 69-73

Contributors

author

F. Aranishi

author

T. Okimoto

author

S. Izumi

• Document Type: ARTICLE
• Publication order reference: F. Aranishi, Marine Molecular Ecology Laboratory, Department of Biological and Environmental Sciences, Miyazaki University, Miyazaki 889-2192, Japan