Glycoforms of six serum glycoproteins in a patient with congenital disorder of glycosylation type I

• Authors: M. Ferens-Sieczkowska , K. Zwierz , A. Midro , I. Katnik-Prastowska
• Languages of publication: EN

Abstracts

EN

In this paper the occurrence and relative content of defectively glycosylated serum glycoforms in transferrin (Tf), 1-acid glycoprotein (AGP), haptoglobin (Hp), 1-antitrypsin (1-AT), 2-macroglobulin (2-MG) and ceruloplasmin (Cpl) in the serum of a patient with congenital disorder of glycosylation type I are reported. Blood samples were taken when the patient was 14 years old and then after a one-year interval. The patterns of glycoforms in both samples were compared. In 4 out of 6 examined glycoproteins, glycoforms lacking one and two oligosaccharide chains occurred. ?Underglycosylated? glycoforms of 2-MG and Cpl were not clearly detectable. Tf was shown to be affected with this defect to a higher extent than other glycoproteins, containing only 30% properly glycosylated molecules and also as much as 30% of the molecules lacking two glycan units. In Hp and 1-AT the proportions of properly and defectively glycosylated forms were similar. This properly glycosylated form comprised 47% of the Hp and 51?55% of the 1-AT molecules. As in AGP and Tf, about 30% the of molecules lacked one glycan unit. Twenty-one percent of the Hp molecules were devoid of two glycans, and this amount slightly increased in the course of the year. In 1-AT, 19 and 17% of the molecules lacked two glycans in both samples, respectively. Only in AGP we did find a substantial difference between the two blood samples. In the course of the year, the amount of the form lacking 2-chains decreased from 12 to 3%, resulting in a simultaneous increase in the forms lacking one chain and the properly glycosylated. Our work also indicates, that applying a simple method of biochemical analysis such as SDS-PAGE/Western-blotting could be helpful in preliminary diagnosis and could improve the identification of congenital disorders of glycosylation.

Keywords

EN

ANTITRYPSIN; CERULOPLASMIN; DISORDER; GLYCOPROTEIN; GLYCOSYLATION; HAPTOGLOBIN; MACROGLOBULIN; TRANSFERRIN

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2002
• Volume: 50
• Issue: 1
• Pages: 67-74

Contributors

author

M. Ferens-Sieczkowska

author

K. Zwierz

author

A. Midro

author

I. Katnik-Prastowska

• Document Type: ARTICLE
• Publication order reference: M.Ferens-Sieczkowska, Deptartment of Chemistry and Immunochemistry, Wroclaw Medical University, Bujwida 44A, 50-345 Wroclaw, Poland