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Number of results

Journal

2010 | 3 | 202-209

Article title

Micropropagation in in vitro culture efficiency of selected protected species Asteraceae

Title variants

Languages of publication

EN

Abstracts

EN
The aim of the present research was the assessment of efficiency of micropropagation system for selected species that belong to Asteraceae family, as well as analysis of morphological traits and plantlets ability to flower. The experimental material were shoot tips isolated from 7-day-old seedlings of Leontopodium alpinum, Senecio macrophyllus, Carlina acaulis, and Cirsium pannonicum. The shoot tips were transferred on the medium supplemented with BA (1 mg.dm-3), and NAA (0,1 mg.dm-3). The obtained shoots were transferred onto fresh medium with the same combination of growth regulator (3 subcultures). The shoots with 4 or more leaves were rooted on the half-strength MS medium without growth regulators for 4 weeks. The plantlets were acclimatized to ex vitro conditions and planted to the field. The analysis of flowering ability, leaves and flower morphology, and survival level were the objectives of the study. The plantlets were acclimatized in a greenhouse and planted to the field condition. From 10 seeds of initial material one can obtain 24 278 plants of Leontopodium alpinum, 1507 of Carlina acaulis, 1261 of Senecio macrophyllus, and 37 of Cirsium pannonicum taking into consideration the percentage of seed germination, proliferation rate in three subcultures, frequency of microshoots rooting and survival rate during acclimatization. The regenerated plants demonstrated traits of donor plants and were able to flower and produce viable seeds.

Journal

Year

Issue

3

Pages

202-209

Physical description

Contributors

author
author

References

Document Type

ARTICLE

Publication order reference

Alina Trejgell, Zaklad Biotechnologii, Instytut Biologii Ogolnej i Molekularnej, Uniwersytet Mikolaja Kopernika w Toruniu, ul Gagarina 9, Torun, Poland

Identifiers

YADDA identifier

bwmeta1.element.element-from-psjc-3c110cf3-c193-3cfe-af4a-9136ab83e473
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