Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays

• Authors: L. Mayur , M. Bochenek , A. Augustznek
• Languages of publication: EN

Abstracts

EN

The cell surface exposure of phosphatidylserine (PS) and the plasma membrane impairment were assessed in the bone marrow of adult male Swiss mice exposed to a single 6 Gy dose of 60Co gamma-rays, and treated intraperitoneally with the aminothiol WR-2721 (Amifostine, S-2-/3-aminopropylamino/ethyl phosphorothioic acid), at a dose of 400 mg/kg body weight, 30 min prior to gamma-irradiation. The bone marrow cells were stained with a combination of fluoresceinated annexin V (annexin V - FITC) and propidium iodide (PI) at 3 h, 7 h, and 24 h after treatment of mice with WR-2721 and 60Co gamma-irradiation. The number of early apoptotic cells (annexin V - FITC positive/ PI negative), and late apoptotic and necrotic cells (annexin V - FITC positive/ PI positive), was increased at 3 h after exposure of mice to 60Co gamma-rays and thereafter declined with the frequency of apoptotic and necrotic cells remaining lower in WR-2721 pre-treated mice. Using the annexin V - FITC flow cytometric assay, the radioprotective effect of WR-2721 against induction of apoptosis and necrosis in normal cells of the haematopoietic system was shown.

Keywords

EN

APOPTOSIS; BONE MARROW; FLOW CYTOMETRY; GAMMA-RAY; MOUSE; NECROSIS

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2002
• Volume: 50
• Issue: 1-2
• Pages: 95-100

Contributors

author

L. Mayur

author

M. Bochenek

author

A. Augustznek

• Document Type: ARTICLE
• Publication order reference: L. Mazur, Department of Animal Physiology, Laboratory of Experimental Haematology and Toxicology, Institute of Zoology, Jagiellonian University, R. Ingardena 6, 30-060 Krakow, Poland