The effect of xylose, galactose and sorbose on the production of selected extracellular enzymes by mutant-strain Trichoderma reesei M-7 during continuous cultivation

• Authors: P. Janas , Targonski P. Zdzislaw , S. Mleko
• Languages of publication: PL

Abstracts

EN

The effect of xylose, galactose and sorbose on the production of cellulases, xylanases, lytic enzymes and beta-galactosidase by mutant ?strain Trichoderma reesei M-7 was analysed. The aforementioned sugars were used separately and in the form of mixtures with lactose as the source of carbon during continuous cultivation of mutant M-7. Feeding medium containing mixtures of xylose and lactose in the ratio of 0,25/0,75% and 0,5/0,5% induced an increased synthesis of xylanases. Increased xylanolytic activity (about 12-27%) with the xylose/glucose ratio of 0,25/0,75% and a significantly increased activity (about 2,5 fold) with the ratio of both sugars of 0,5/0,5% was observed in comparison with continuous cultivation in the presence of 1% of lactose in the medium. Reduced synthesis of all cellulolytic (FPU, ?-1,4-endoglucanase, ?-glucosidase) and xylanolytic enzymes correlated with the increased galactose concentration in the feeding medium. Galactose also repressed the production of ?-galactosidase of the mutant-strain M-7. The results obtained after continuous cultivation with sorbose and lactose showed that sorbose was a worse inducer of synthesis of cellulolytic enzymes than lactose.

Keywords

EN

CELLULASE; LYTIC ENZYME; TRICHODERMA REESEI; XYLANASE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2002
• Issue: 1
• Pages: 195-207

Contributors

author

P. Janas

author

Targonski P. Zdzislaw

author

S. Mleko

• Document Type: ARTICLE
• Publication order reference: P. Janas, Katedra Technologii Przemyslu Rolno-Spozywczego i Przechowalnictwa. Akademia Rolnicza, ul. Skromna 8, 20-950 Lublin, Poland