Haploidy in early bovine embryos produced in vitro

• Authors: D. Lechniak
• Languages of publication: EN

Abstracts

EN

Early bovine embryos (two -to sixteen blastomers) produced in vitro were cytogenetically analysed to determine the incidence of haploidy.Follicular oocytes were matured in vitro and inseminated with sperm prepared using the swim up method.After 2-3 days of culture, chromosome slides were prepared according to the air-drying technique and stained with Giemsa.Altogether 202 embryos produced mathaphase spreads.Of these 4.5% were pure haploid embryos and 5.5% displayed a haploid cell line within mosaic embryos (n/2n and n/3n).The occurence of haploid embryos observed in this study was compared to results of other studies and a possible origin of haploidy was discussed.

Keywords

EN

BOVINE EMBRYO; HAPLOIDY; IN VITRO FERTILIZATION; MIXOPLOIDY

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1995
• Volume: 36
• Issue: 4
• Pages: 363-371

Contributors

author

D. Lechniak

• Document Type: article
• Publication order reference: D.Lechniak