Plastids in callus tissue culture of Plantago asiatica L.

• Authors: J. Makowczynska , E. Andrzejewska-Golec , K. Marek
• Languages of publication: PL

Abstracts

EN

For the last few years, in the Department of Biology and Pharmaceutical Botany ? Plantago asiatica L. ? a medicinal plant, popular in the Far East, has been cultured in vitro. Two lines of the nonmorphogenic callus were obtained: cream- and green- coloured. They were induced from the roots taken from 4-week-old seedlings. The cream-coloured callus was cultured on Murashige and Skoog medium enriched with 0.5 mg/dm3 2,4- D and 0.2 mg/dm3 BAP. The green callus was initiated on the same medium supplemented with 0.1 mg/dm3 IAA and 2 mg/dm3 BAP and next it was cultured on MS with 0.5 mg/dm3 IAA and 0.2 mg/dm3 BAP. The fragments of the callus were studied using the light microscope and the transmission electron microscope. For cream-coloured callus, proplastids and amyloplasts were observed. In green callus, in addition to the plastids found in cream-coloured callus, also chloroplasts were observed.

Keywords

EN

CALLUS CULTURE; PLANTAGO ASIATICA; PLASTID

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 275-281

Contributors

author

J. Makowczynska

author

E. Andrzejewska-Golec

author

K. Marek

• Document Type: ARTICLE
• Publication order reference: E. Andrzejewska-Golec, Zaklad Biologii i Botaniki Farmaceutycznej, Uniwersytet Medyczny, ul. Muszynskiego 1, 90-151 Lodz, Poland