Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum

• Authors: P. Venkataiah , T. Christopher , K. Subhash
• Languages of publication: EN

Abstracts

EN

Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The explants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L?1 IAA and 500 mg L?1 streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.

Keywords

EN

CAPSICUM PRAETERMISSUM; COTYLEDON; INHERITANCE; MUTAGENESIS; RESISTANCE; STREPTOMYCIN

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2005
• Volume: 46
• Issue: 1
• Pages: 19-24

Contributors

author

P. Venkataiah

author

T. Christopher

author

K. Subhash

• Document Type: ARTICLE
• Publication order reference: P. Venkataiah, Plant Biotechnology Laboratory, Department of Botany, Kakatiya University, Warangal?506 009 A P, India