The aim of the present work was to evaluate the morphological and physiological status of strawberry shoots (cv. Senga Sengana) cultivated in vitro and their subsequent (out of glass vessels) ability to form plantlets with developed autothrophic metabolism. Standard medium recommended by Boxus was supplemented with glucose or sucrose 30 g/l. Biomass production and particular shoot formation were more efficient in the presence of glucose. The capacity of the shoots to form the root system and to develop photosynthetic activity was higher for shoots taken from the glucose-medium than the sucrose containing medium.