EN
Spontaneous parthenogenetic activation of bovine oocytes in an in vitro maturation and fertilization system (IVM/IVF) is described. Altogether, 1403 follicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10%) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with a minority (15.7%) comprising of 9-16 blastomeres. According to a cytogenetic analysis, only 1.2% of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryos produced in vitro and spontaneous parthenogenetic activation in an IVM/IVF system indicates suboptimal culture conditions. A group of non-inseminated oocytes should be included in each experiment to serve as a control. Spontaneously activated bovine parthenotes only occasionaly developed beyond the 8-blastomere stage in a common IVM/IVF system. The incidence of parthenotes interferes with the efficiency of in vitro embryo production but it is doubtful whether it lowers the pregnancy rate after transfer of IVF embryos.