Assessment of DNA damage profile and oxidative /antioxidative biomarker level in patients with inflammatory bowel disease

• Authors: Małgorzata Mrowicka , Jerzy Mrowicki , Michał Mik , Łukasz Dziki , Adam Dziki , Ireneusz Majsterek

Abstracts

EN

Aim: The purpose of this study was to investigate the oxidative DNA damage, pro-antioxidant status in Polish patients with inflammatory bowel disease (IBD).
Methods: Oxidative DNA damage was measured by comet assay techniques; nitric oxide (NO) and plasmatic lipid peroxidation (MDA) as oxidative stress were valuated by colometric methods; superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx1) as antioxidative defense were determined by spectrophotometric methods.
Results: The level of oxidative DNA damage in IBD patients was significantly higher in relation to controls (P = 0.01). Alike, in control subject as well as in patients with IBD, lymphocytes are characterized by complete repair of DNA damage. A significant decrease of SOD (P = 0.031), CAT (P = 0.006), GPx1 (P = 0.001) activity was seen in IBD patients vs control. MDA (P = 0.001) and NO (P = 0.001) concentrations were significantly increased in IBD patients as compared to healthy subjects.
Conclusions: Our results may be due to the induction of DNA repair genes which may occur at the stage of the pathological changes (IBD) that may be caused by excessive oxidative stress. However, the cause of this relationship, and whether it is direct or indirect, remains to be explored.

Keywords

EN

DNA oxidative damage repair; inflammatory bowel diseases; pro/antioxidant status

• Publisher: Index Copernicus International
• Journal: Polish Journal of Surgery
• Year: 2020
• Volume: 92(5)
• Pages: 8-14

Dates

published

2020-05-26

Contributors

author

Małgorzata Mrowicka

author

Jerzy Mrowicki

author

Michał Mik

author

Łukasz Dziki

author

Adam Dziki

author

Ireneusz Majsterek

Identifiers

DOI

10.5604/01.3001.0014.1548