Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy

• Authors: J. Kowalska , M. Gajda , W. Kwiatek , M. Franczyk-Żarów , R. Kostogrys , S. Chłopicki , C. Sandt , P. Dumas
• Languages of publication: EN

Abstracts

EN

Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/LDLR^{-/-} mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids (CH_2, CH_3 bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 cm^{-1}/1656 cm^{-1} attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 cm^{-1}) was observed between egg-rich diet fed animals and the control group.

Keywords

EN

87.19.xr; 87.64.km; 87.64.-t

Discipline

• 87.64.km: Infrared
• 87.64.-t: Spectroscopic and microscopic techniques in biophysics and medical physics
• 87.19.xr: Degenerative diseases (Alzheimer's, ALS, etc)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2012
• Volume: 121
• Issue: 2
• Pages: 555-560

Dates

published

2012-02

Contributors

author

J. Kowalska

• The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, Poland

author

M. Gajda

• Department of Histology, Jagiellonian University Medical College, Kraków, Poland

author

W. Kwiatek

• The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, Poland

author

M. Franczyk-Żarów

• Faculty of Food Technology, Department of Human Nutrition, Agricultural University, Kraków, Poland

author

R. Kostogrys

• Faculty of Food Technology, Department of Human Nutrition, Agricultural University, Kraków, Poland

author

S. Chłopicki

• Department of Experimental Pharmacology, Jagiellonian University Medical College, Kraków, Poland

author

C. Sandt

• French Synchrotron Facility SOLEIL, Gif-sur-Yvette Cedex, France

author

P. Dumas

• French Synchrotron Facility SOLEIL, Gif-sur-Yvette Cedex, France

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Identifiers

DOI

10.12693/APhysPolA.121.555