E2F site in the essential promoter region does not confer S phase-specific transcription of the ABCC10 gene in human prostate cancer cells

• Authors: Magdalena Dabrowska , Francis Sirotnak
• Languages of publication: EN

Abstracts

EN

ABCC10 (MRP7) plays a role in cellular detoxification and resistance to anticancer drugs. Since ABCC10 gene transcription in human prostate cancer CWR22Rv1 cells was found dependent on E2F binding sequence motif, ABCC10 expression in G1 and S phases of the cell cycle of CWR22Rv1 cells, was analyzed. The cells were synchronized in G1 phase by double thymidine block and in S phase by thymidine/mimosine double block. ABCC10 mRNA level was found to be similar in S phase-synchronized and asynchronous cell populations. In G1 phase it decreased by 2.4- to 3-fold. It is thus inferred, that ABCC10 expression in CWR22Rv1 cells is not S phase-specific but is primarily associated with cell proliferation.

Keywords

EN

ABCC10; MRP7; E2F; p107; RBL1; cell cycle; non-classical E2F target gene

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 371-374

Dates

published

2017

received

2017-02-01

revised

2017-03-28

accepted

2017-03-29

(unknown)

2017-06-13

Contributors

author

Magdalena Dabrowska

• Laboratory of Molecular Basis of Ageing, Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Francis Sirotnak

• Memorial Sloan-Kettering Cancer Center, New York, NY10021, USA.

References

• Andrusiak MG, McClellan KA, Dugal-Tessier D, Julian LM, Rodrigues SP, Park DS, et al. (2011) Rb/E2F regulates expression of neogenin during neuronal migration. Mol Cell Biol 31: 238-247. doi: 10.1128/MCB.00378-10.
• Bessho Y, Oguri T, Ozasa H, Uemura T, Sakamoto H, Miyazaki M, et al. (2009) ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer. Oncol Rep 21: 263-268. doi: 10.3892/or_00000217.
• Bracken AP, Ciro M, Cocito A, Helin K (2004) E2F target genes: unraveling the biology. Trends Biochem Sci 29: 409-417. doi: 10.1016/j.tibs.2004.06.006.
• Chen ZS, Hopper-Borge E, Belinsky MG, Shchaveleva I, Kotova E, Kruh GD (2003) Characterization of the transport properties of human multidrug resistance protein 7 (MRP7, ABCC10). Mol Pharmacol 63: 351-358. doi: 10.1124/mol.63.2.351.
• Chung LC, Tsui KH, Feng TH, Lee SL, Chang PL, Juang HH (2012) L-Mimosine blocks cell proliferation via upregulation of B-cell translocation gene 2 and N-myc downstream regulated gene 2 in prostate carcinoma cells. Am J Physiol Cell Physiol 302: C676-C685. doi: 10.1152/ajpcell.00180.2011.
• Dabrowska M, Sirotnak FM (2004) Regulation of transcription of the human MRP7 gene. Characteristics of the basal promoter and identification of tumor-derived transcripts encoding additional 5' end heterogeneity. Gene 341: 129-139. doi: 10.1016/j.gene.2004.06.022.
• Dimova DK, Dyson NJ (2005) The E2F transcriptional network: old acquaintances with new faces. Oncogene 24: 2810-2826.
• Henley SA, Dick FA (2012) The retinoblastoma family of proteins and their regulatory functions in the mammalian cell division cycle. Cell Division 7: 10. doi: 10.1038/sj.onc.1208612.
• Hopper E, Belinsky MG, Zeng H, Tosolini A, Testa JR, Kruh GD (2001) Analysis of the structure and expression pattern of MRP7 (ABCC10), a new member of the MRP subfamily. Cancer Lett 162: 181-191. doi: 10.1016/S0304-3835(00)00646-7.
• Hopper-Borge EA, Churchill T, Paulose C, Nicolas E, Jacobs JD, Ngo O, et al. (2011) Contribution of Abcc10 (Mrp7) to in vivo paclitaxel resistance as assessed in Abcc10-/- mice. Cancer Res 71: 3649-3657. doi: 10.1158/0008-5472.CAN-10-3623.
• Hopper-Borge EA, Xu X, Shen T, Shi Z, Chen ZS, Kruh GD (2009) Human multidrug resistance protein 7 (ABCC10) is a resistance factor for nucleoside analogs and epothilone B. Cancer Res 69: 178-184. doi: 10.1158/0008-5472.CAN-08-1420.
• Julian LM, Blais A (2015) Transcriptional control of stem cell fate by E2Fs and pocket proteins. Front Genet 6: 161. doi: 10.3389/fgene.2015.00161.
• Julian LM, Pakenham CA, Dugal-Tessier D, Ruzhynsky V, Bae S, et al. (2016) Tissue-specific targeting of cell fate regulatory genes by E2f factors. Cell Death Differ 23: 565-575. doi: 10.1038/cdd.2015.36.
• Karwaciak I, Pulaski L, Ratajewski M (2014) Regulation of the human ABCB10 gene by E2F transcription factors. Genomics 104: 520-529. doi: 10.1016/j.ygeno.2014.08.022.
• Kathawala RJ, Gupta P, Ashby CR Jr, Chen ZS (2015) The modulation of ABC transporter-mediated multidrug resistance in cancer: A review of the past decade. Drug Resist Updat 18: 1-17. doi: 10.1016/j.drup.2014.11.002.
• Khokhar NZ, She Y, Rusch VW, Sirotnak FM (2001) Experimental therapeutics with a new 10-deazaaminopterin in human mesothelioma: further improving efficacy through structural design, pharmacologic modulation at the level of MRP ATPases, and combined therapy with platinums. Clin Cancer Res 7: 3199-3205.
• Krude T (1999) Mimosine arrests proliferating human cells before onset of DNA replication in a dose-dependent manner. Exp Cell Res 247: 148-159. doi: 10.1006/excr.1998.4342.
• Li J, Xuan JW, Khatamianfar V, Valiyeva F, Moussa M, Sadek A, et al. (2014) SKA1 over-expression promotes centriole over-duplication, centrosome amplification and prostate tumorigenesis. J Pathol 234: 178-189. doi: 10.1002/path.4374.
• Oguri T, Ozasa H, Uemura T, Bessho Y, Miyazaki M, Maeno K, et al. (2008) MRP7/ABCC10 expression is a predictive biomarker for the resistance to paclitaxel in non-small cell lung cancer. Mol Cancer Ther 7: 1150-1155. doi: 10.1158/1535-7163.MCT-07-2088.
• Rosner M, Schipany K, Hengstschlager M (2013) Merging high-quality biochemical fractionation with a refined flow cytometry approach to monitor nucleocytoplasmic protein expression throughout the unperturbed mammalian cell cycle. Nat Protocols 8: 602-626. doi: 10.1038/nprot.2013.011.
• Ryo A, Liou YC, Wulf G, Nakamura M, Lee SW, Lu KP (2002) PIN1 is an E2F target gene essential for Neu/Ras-induced transformation of mammary epithelial cells. Mol Cell Biol 22: 5281-5295. doi: 10.1128/MCB.22.15.5281-5295.2002.
• Sosa-Garcia B, Vazquez-Rivera V, Gonzalez-Flores JN, Engel BE, Cress WD, Santiago-Cardona PG (2015) The retinoblastoma tumor suppressor transcriptionally represses Pak1 in osteoblasts. PLoS One 10: e0142406. doi: 10.1371/journal.pone.0142406.
• Spector DL, Goldman RD, Leinwand LA (1998) Cells: a laboratory manual. Cold Spring Harbor Laboratory Press, Long Island, NY.
• Wang LY, Hung CL, Chen YR, Yang JC, Wang J, Campbell M, et al. (2016) KDM4A coactivates E2F1 to regulate the PDK-dependent metabolic switch between mitochondrial oxidation and glycolysis. Cell Rep 16: 3016-3027. doi: 10.1016/j.celrep.2016.08.018.
• Wartenberg M, Fischer K, Hescheler J, Sauer H (2002) Modulation of intrinsic P-glycoprotein expression in multicellular prostate tumor spheroids by cell cycle inhibitors. Biochim Biophys Acta 1589: 49-62. doi: 10.1016/S0167-4889(01)00185-9.
• Xu X, Bieda M, Jin VX, Rabinovich A, Oberley MJ, Green R, Farnham PJ (2007) A comprehensive ChIP-chip analysis of E2F1, E2F4 and E2F6 in normal and tumor cells reveals interchangeable roles of E2F family members. Genome Res 17: 1550-1651. doi: 10.1101/gr.6783507.

Identifiers

DOI

10.18388/abp.2017_1521