The use of serine protease from Yarrowia lipolytica yeast in the production of biopeptides from denatured egg white proteins

• Authors: Marta Pokora , Aleksandra Zambrowicz , Agnieszka Zabłocka , Anna Dąbrowska , Marek Szołtysik , Konrad Babij , Ewelina Eckert , Tadeusz Trziszka , Józefa Chrzanowska
• Languages of publication: EN

Abstracts

EN

Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w). The obtained hydrolysate was characterized by protein degradation of 38% and also exhibited an antioxidant and cytokine-inducing activity. The isolation procedure (ultrafiltration and RP-HPLC) of bioactive peptides from the EP hydrolysate provided peptide fractions with significant antioxidant and ACE inhibitory activities. Three homogeneous and three heterogeneous peptide fractions were identified using MALDI-TOF/MS and the Mascot Search Results database. The peptides, mainly derived from ovalbumin, were composed of 2-19 amino-acid residues. We have thus demonstrated a novel ability of serine protease from Y. lipolytica to release biopeptides from an EP by-product.

Keywords

EN

Yarrowia lipolytica; serine protease; bioactive peptide; antioxidant; ACE-inhibitor

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 245-253

Dates

published

2017

received

2016-05-12

revised

2016-09-29

accepted

2016-10-17

(unknown)

2017-04-07

Contributors

author

Marta Pokora

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Aleksandra Zambrowicz

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Agnieszka Zabłocka

• Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Anna Dąbrowska

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Marek Szołtysik

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Konrad Babij

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Ewelina Eckert

• Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada

author

Tadeusz Trziszka

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

author

Józefa Chrzanowska

• Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

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Identifiers

DOI

10.18388/abp.2016_1316