The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system

• Authors: Aurelia Piekarska , Paulina Pełka , Grażyna Peszyńska-Sularz , Alessandro Negri , Krzysztof Hinc , Michał Obuchowski , Adam Iwanicki
• Languages of publication: EN

Abstracts

EN

The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.

Keywords

EN

Bacillus subtilis; spore display; APCs; FliD; Clostridium difficile; immune response

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 239-244

Dates

published

2017

received

2016-09-25

revised

2016-10-12

accepted

2016-10-17

(unknown)

2017-04-12

Contributors

author

Aurelia Piekarska

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland

author

Paulina Pełka

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland

author

Grażyna Peszyńska-Sularz

• Tri-City Animal Laboratory, Medical University of Gdańsk, Gdańsk, Poland

author

Alessandro Negri

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland

author

Krzysztof Hinc

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

author

Michał Obuchowski

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

author

Adam Iwanicki

• Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland

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Identifiers

DOI

10.18388/abp.2016_1315