Age-dependent systemic DNA damage in early Type 2 Diabetes mellitus

• Authors: Dinko Rogulj , Ismail El Aklouk , Paško Konjevoda , Spomenka Ljubić , Mirjana Pibernik Okanović , Ante Barbir , Marijana Luburić , Maja Radman , Ninoslav Budinski , Marijana Vučić Lovrenčić
• Languages of publication: EN

Abstracts

EN

Oxidative stress, capable of eliciting damage to various biomolecules including DNA, is a recognized component of diabetes mellitus and its complications. Metabolic syndrome (MetS) is associated with the development of type 2 diabetes mellitus (T2DM), as well as other unfavorable outcomes. The aim of this study was to elucidate the role of oxidative stress in the development of T2DM, by investigating association of oxidative DNA damage with metabolic parameters in subjects with MetS and early T2DM. Selected anthropometric and biochemical parameters of MetS, inflammation and oxidative DNA damage: body mass index (BMI), fatty liver index (FLI), waist circumference (WC), total cholesterol, HDL and LDL-cholesterol, gamma-glutamyl transpeptidase (GGT), uric acid, C-reactive protein (CRP), total leukocyte/neutrophil count, and urinary 8-hidroxy-deoxyguanosine (u-8-OHdG) were assessed in male subjects with MetS and both younger (≤55 years) and older (>55 years) subjects with T2DM of short duration without complications. BMI, FLI, WC, total and LDL-cholesterol and uric acid were higher, while the u-8-OHdG was lower in MetS group, when compared to older T2DM subjects. None of these parameters were different neither between MetS and younger T2DM, nor between two sub-groups of subjects with T2DM. Values of CRP, HDL-cholesterol, triglycerides, GGT, leukocytes and neutrophils were not different between all examined groups of subjects. Higher 8-OHdG in older subjects with T2DM suggests that both aging process and diabetes could contribute to the development of DNA damage. Oxidative DNA damage cannot serve as an universal early marker of T2DM.

Keywords

EN

metabolic syndrome; type 2 diabetes mellitus; DNA damage; urinary 8-OHdG

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2017
• Volume: 64
• Issue: 2
• Pages: 233-238

Dates

published

2017

received

2016-05-05

revised

2016-07-12

accepted

2016-10-06

(unknown)

2017-03-30

Contributors

author

Dinko Rogulj

• Department of Internal Medicine, General Hospital Brežice, Slovenia

author

Ismail El Aklouk

• General Hospital Varaždin, Department for extending and palliative care Novi Marof, Croatia

author

Paško Konjevoda

• NMR Centre, Institute Rudjer Bošković, Zagreb, Croatia

author

Spomenka Ljubić

• Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, University Hospital Merkur, Zagreb, Croatia

author

Mirjana Pibernik Okanović

• Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, University Hospital Merkur, Zagreb, Croatia

author

Ante Barbir

• Polyclinic Sunce, Zagreb, Croatia

author

Marijana Luburić

• Polyclinic Sunce, Zagreb, Croatia

author

Maja Radman

• Department of Internal Medicine, University Hospital Centre Split, Split, Croatia

author

Ninoslav Budinski

• Department of Internal Medicine, University Hospital Sveti Duh, Zagreb, Croatia

author

Marijana Vučić Lovrenčić

• Department of Laboratory Medicine, Scientific Research Unit, University Hospital Merkur, Zagreb, Croatia

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Identifiers

DOI

10.18388/abp.2016_1313