Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

• Authors: Marcin Grąz , Kamila Rachwał , Radosław Zan , Anna Jarosz-Wilkołazka
• Languages of publication: EN

Abstracts

EN

Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of Km for oxalate and Vmax for the enzyme reaction were 0.015 M and 30 mmol min-1, respectively.

Keywords

EN

oxalate oxidase; oxalic acid; Abortiporus biennis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2016
• Volume: 63
• Issue: 3
• Pages: 595-600

Dates

published

2016

received

2016-03-04

revised

2016-04-25

accepted

2016-05-20

(unknown)

2016-06-23

Contributors

author

Marcin Grąz

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

author

Kamila Rachwał

• Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland

author

Radosław Zan

• Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland

author

Anna Jarosz-Wilkołazka

• Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

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Identifiers

DOI

10.18388/abp.2016_1282