Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly

• Authors: Donia Mili , Dane Georgesse , Abderraouf Kenani
• Languages of publication: EN

Abstracts

EN

The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated Protein 55kDa (RAP55/LSM14) during mitosis was identified as a Mitotic Spindle Protein (MSP). We found a new location obtained by expressing GFP-tagged proteins in HeLa Cells during mitosis that has never been previously reported. We demonstrated also, for the first time, that the depletion of RAP55/LSM14 destabilizes spindle assembly, stops cells in mitosis and induces many other cell cytoskeletal disorders. Finally, by using an "in vitro" assay investigation, we found that RAP55/LSM14 binds directly the tubulin and that is implicated in the process of the mitotic spindle stabilization, which is a novel discovery in this field of research.

Keywords

EN

LSM14; mitosis; microtubule; mRNP; RAP55; mitotic spindle

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2015
• Volume: 62
• Issue: 3
• Pages: 613-619

Dates

published

2015

received

2015-07-11

revised

2015-08-10

accepted

2015-08-11

(unknown)

2015-09-04

Contributors

author

Donia Mili

• Inserm Unité 836, 38043 Grenoble, France
• UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie

author

Dane Georgesse

• Inserm Unité 836, 38043 Grenoble, France

author

Abderraouf Kenani

• UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie

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Identifiers

DOI

10.18388/abp.2015_1107