Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector

• Authors: Guohui Li , Mangmang Li , Peng Wang , Zhaoyang Hu , Qin Yao , Qi Tang , Keping Chen
• Languages of publication: EN

Abstracts

EN

ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.

Keywords

EN

BmBDV; NS1; baculovirus expression vector; Sf9; egfp

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 4
• Pages: 787-794

Dates

published

2014

received

2014-05-19

revised

2014-10-07

accepted

2014-10-11

(unknown)

2014-11-06

Contributors

author

Guohui Li

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Mangmang Li

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Peng Wang

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Zhaoyang Hu

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Qin Yao

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Qi Tang

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

author

Keping Chen

• Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China

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