A computational approach to structural properties of glycoside hydrolase family 4 from bacteria

• Authors: Dana Craciun , Beatrice Vlad-Oros , Nicoleta Filimon , Vasile Ostafe , Adriana Isvoran
• Languages of publication: EN

Abstracts

EN

Structural bioinformatics approaches applied to the alpha- and beta-glycosidases from the GH4 enzyme family reveal that, despite low sequence identity, these enzymes possess quite similar global structural characteristics reflecting a common reaction mechanism. Locally, there are a few distinctive structural characteristics of GH4 alpha- and beta-glycosidases, namely, surface cavities with different geometric characteristics and two regions with highly dissimilar structural organizations and distinct physicochemical properties in the alpha- and beta-glucosidases from Thermotoga maritima. We suggest that these structurally dissimilar regions may be involved in specific protein-protein interactions and this hypothesis is sustained by the predicted distinct functional partners of the investigated proteins. Also, we predict that alpha- and beta-glycosidases from the GH4 enzyme family interact with difenoconazole, a fungicide, but there are different features of these interactions especially concerning the identified structurally distinct regions of the investigated proteins.

Keywords

EN

glycosidases; structural properties; specific interactions

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 553-564

Dates

published

2013

received

2013-01-27

revised

2013-10-02

accepted

2013-12-06

(unknown)

2013-12-16

Contributors

author

Dana Craciun

• West University of Timisoara, Teacher Training Department, Timisoara, Romania

author

Beatrice Vlad-Oros

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania

author

Nicoleta Filimon

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania

author

Vasile Ostafe

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania

author

Adriana Isvoran

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania

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