A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases

• Authors: Beata Podgórska , Grażyna Kujawska , Michał Skurzewski , Olesya Batsko , Tadeusz Kaczorowski
• Languages of publication: EN

Abstracts

EN

In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.

Keywords

EN

site-specific endonuclease purification; type II restriction endonuclease; bacteria screening

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 4
• Pages: 669-672

Dates

published

2012

received

2012-07-23

revised

2012-10-16

accepted

2012-10-29

(unknown)

2012-11-06

Contributors

author

Beata Podgórska

• Department of Molecular Evolution, University of Gdańsk, Gdańsk, Poland

author

Grażyna Kujawska

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

author

Michał Skurzewski

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

author

Olesya Batsko

author

Tadeusz Kaczorowski

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

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