Effect of substrate stiffness on differentiation of umbilical cord stem cells

• Authors: Małgorzata Witkowska-Zimny , Katarzyna Walenko , Anna Ewa Wałkiewicz , Zygmunt Pojda , Jacek Przybylski , Małgorzata Lewandowska-Szumieł
• Languages of publication: EN

Abstracts

EN

Tissue formation and maintenance is regulated by various factors, including biological, physiological and physical signals transmitted between cells as well as originating from cell-substrate interactions. In our study, the osteogenic potential of mesenchymal stromal/stem cells isolated from umbilical cord Wharton's jelly (UC-MSCs) was investigated in relation to the substrate rigidity on polyacrylamide hydrogel (PAAM). Osteogenic differentiation of UC-MSCs was enhanced on stiff substrate compared to soft substrates, illustrating that the mechanical environment can play a role in differentiation of this type of cells. These results show that substrate stiffness can regulate UC-MSCs differentiation, and hence may have significant implications for design of biomaterials with appropriate mechanical properties for regenerative medicine.

Keywords

EN

substrate stiffness; osteogenic differentiation; umbilical cord mesenchymal stromal/stem cells

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 2
• Pages: 261-264

Dates

published

2012

received

2011-10-17

revised

2012-01-03

accepted

2012-04-04

(unknown)

2012-05-11

Contributors

author

Małgorzata Witkowska-Zimny

• Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland

author

Katarzyna Walenko

• Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland

author

Anna Ewa Wałkiewicz

• Institute of Physics, Polish Academy of Sciences, Warszawa, Poland

author

Zygmunt Pojda

• Department of Regenerative Medicine, WIHE Institute of Hygiene and Epidemiology

author

Jacek Przybylski

• Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland

author

Małgorzata Lewandowska-Szumieł

• Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland

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