The mitochondrial complex II and ATP-sensitive potassium channel interaction: quantitation of the channel in heart mitochondria

• Authors: Andrew P Wojtovich , Keith W Nehrke , Paul S Brookes
• Languages of publication: EN

Abstracts

EN

The mitochondrial ATP-sensitive potassium channel (mKATP) is important in cardioprotection, although the channel remains molecularly undefined. Several studies have demonstrated that mitochondrial complex II inhibitors activate the mKATP, suggesting a potential role for complex II in channel composition or regulation. However, these inhibitors activate mKATP at concentrations which do not affect bulk complex II activity. Using the potent complex II inhibitor Atpenin A5, this relationship was investigated using tight-binding inhibitor theory, to demonstrate that only 0.4 % of total complex II molecules are necessary to activate the mKATP. These results estimate the mKATP content at 15 channels per mitochondrion.

Keywords

EN

atpenin A5; complex II; mKATP; ischemic preconditioning

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 4
• Pages: 431-434

Dates

published

2010

received

2010-08-31

revised

2010-10-31

accepted

2010-11-15

(unknown)

2010-11-19

Contributors

author

Andrew P Wojtovich

• Department of Anesthesiology, University of Rochester Medical Center, Rochester, NY, USA

author

Keith W Nehrke

• Department of Medicine, University of Rochester Medical Center, Rochester, NY, USA

author

Paul S Brookes

• Department of Anesthesiology, University of Rochester Medical Center, Rochester, NY, USA

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