Herpesviruses as possible cofactors in HPV-16-related oncogenesis*

• Authors: Slawa Szostek , Barbara Zawilinska , Jolanta Kopec , Magdalena Kosz-Vnenchak
• Languages of publication: EN

Abstracts

EN

Cervical carcinogenesis is a complex problem with papillomavirus widely accepted as a causative agent. Integration of a human papillomavirus (HPV) of the high-risk type into the host cell genome is one of the major contributing factors to cervical malignant transformation. In this study, the correlation of CMV, EBV, HSV-1, HSV-2, HHV-6 and HHV-7 infections with the physical status of the HPV genome in cervical cancer and precancerous cervical lesions was investigated in sixty HPV-16-positive women. Cervical secretion samples were submitted to DNA extraction and analyzed by PCR. HPV-16 DNA was confirmed in genotyping with the reverse hybridization line probe assay. Multiplex PCR with specific primers for the E2/E6 genes was used to assess the viral integration status of HPV-16. Our results show that CMV DNA was more frequently present in samples with mixed forms of HPV-16 than in the episomal form (P < 0.025). Such a correlation was also observed in the case of EBV (P < 0.005). The presence of CMV resulted in a six-fold (OR 6.069; 95% CI 1.91-19.22; P = 0.002), while EBV caused a seven-fold (OR 7.11; 95% CI 1.70-29.67; P = 0.007) increase in the risk of the integrated or mixed HPV-16 genome occurrence. Our data suggest that coinfection with herpesviruses, especially CMV and EBV, may be involved in the integration of the HPV-16 genome and may contribute to the development of cervical cancer.

Keywords

EN

human papillomavirus; cervical carcinoma; squamous intraepithelial lesions; herpesviruses

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2009
• Volume: 56
• Issue: 2
• Pages: 337-342

Dates

published

2009

received

2009-04-14

revised

2009-05-05

accepted

2009-05-28

(unknown)

2009-06-04

Contributors

author

Slawa Szostek

• Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Barbara Zawilinska

• Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

author

Jolanta Kopec

• Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

author

Magdalena Kosz-Vnenchak

• Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland

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