In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide

• Authors: Rammohan Subramanian , M Asmawi , Amirin Sadikun
• Languages of publication: EN

Abstracts

EN

There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculata extract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.

Keywords

EN

α-glucosidase; α-amylase; Andrographis paniculata; andrographolide; peak blood glucose; post prandial hyperglycaemia.

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 2
• Pages: 391-398

Dates

published

2008

received

2007-11-05

revised

2008-04-03

accepted

2008-04-29

(unknown)

2008-05-29

Contributors

author

Rammohan Subramanian

• Department of Pharmacology, School of Pharmacy, Universiti Sains Malaysia, Penang, Malaysia

author

M Asmawi

• Department of Pharmacology, School of Pharmacy, Universiti Sains Malaysia, Penang, Malaysia

author

Amirin Sadikun

• Department of Pharmaceutical Chemistry, School of Pharmacy, Universiti Sains Malaysia, Penang, Malaysia

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