Changes in the content of γ-linolenic C18:3 (n-6) and stearidonic C18:4 (n-3) acids in developing seeds of viper's bugloss Echium vulgare L.

• Authors: Andrzej Stolyhwo , Jolanta Mol
• Languages of publication: EN

Abstracts

EN

Changes in the composition of fatty acids (FA) were determined in lipid extracts isolated from developing ovaries containing ovules and developing seeds of Echium vulgare L. The samples were collected successively over 20 days beginning with the first day after flowering. The contents of the n-6 FA family members, i.e., γ-linolenic (GLA) (C18:3) and linoleic (LA) (C18:2) acids changed in a parallel manner and reached the maximum of 13.9% and 24%, respectively, on the 12th day, after which they fell systematically down to 8.6% and 18.2%, respectively, on the 20th day after flowering. Starting with day 13, the content of α-linolenic acid (ALA) (C18:3 n-3) begins to grow intensively, from 24.2% to 39.3% on the 20th day after flowering. The increase in the content of stearidonic acid (SDA) (C18:4 n-3), up to 10.5% on the 20th day after flowering, occurred steadily as the seeds developed, and was independent of the changes in the content of GLA and LA. The pattern of changes in the content of SDA, GLA, LA and ALA during the development of seeds, and the occurrence of SDA in the seed oil of other plants, demonstrate that the biosynthesis of SDA in the seeds is critically dependent on the presence of ALA. The above condition indicates that SDA biosynthesis in the seeds of Echium vulgare follows the scheme LA → simultaneous, competitive, action of Δ6 and Δ15 desaturases, leading to the formation of GLA and ALA, respectively, and then ALA (Δ6 des) → SDA. The biosynthesis according to the scheme: GLA (Δ15 des) → SDA is highly unlikely.

Keywords

EN

octadecatetraenoic acid; γ-linolenic acid; lipids; Echium vulgare L.; Boraginaceae; seeds; stearidonic acid; viper's bugloss

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 4
• Pages: 741-746

Dates

published

2007

received

2007-07-30

accepted

2007-11-19

revised

2007-11-19

(unknown)

2007-12-09

Contributors

author

Andrzej Stolyhwo

• Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences (SGGW), Warszawa, Poland

author

Jolanta Mol

• Konopnicka Grammar School, Katowice, Poland

References

• Griffiths G, Brechany E, Jackson FM, Christie W, Stymne S, Stobart KA (1996) Distribution and biosynthesis of stearidonic acid in leaves of Borago officinalis. Phytochemistry 43: 381-386.
• Guil-Guerrero JL, Gómez-Mercado F, Garcia-Maroto F, Campra-Madrid P (2000) Occurrence and characterization of oils rich in γ-linolenic acid. Part I: Echium seeds from Macaronesia. Phytochemistry 53: 451-456.
• Guil-Guerrero JL, Gomez-Mercado F, Rodriguez-Garcia I, Campra-Madrid P, Garcia-Maroto F (2001) Occurrence and characterization of oils rich in γ-linolenic acid (III): the taxonomical value of the fatty acids in Echium (Boraginaceae). Phytochemistry 58: 117-120.
• Gunstone FD (1992) γ-Linolenic acid. Occurrence and physical and chemical properties. Prog Lipid Res 31: 145-161.
• Horrobin DF (1993) Fatty acids metabolism in health and disease: the role of delta-6 desaturase. Am J Clin Nutr 57 (Suppl 5): 732-737.
• Jankowski WJ, Stolyhwo A (1995) Unusual fatty acid composition of cuticular lipids from leaves of Oenothera. J Plant Physiol 145: 215-220.
• Mol J, Rostański K, Szulik J (2001) The Oenothera species evaluation by gas chromatography. Riv Ital Sostanze Grasse 78: 35-40.
• Rostański K (1963) Echium L., Żmijowiec. In: Flora Polska X. Pawłowski B ed, pp 156-161. PWN, Warszawa, Kraków (in Polish).
• Sayanova O, Napier JA, Shewry PR (1999) Delta-6 Unsaturated fatty acids in species and tissues of the Primulaceae. Phytochemistry 52: 419-422.
• Traitler H, Winter H, Richli U, Ingenbleek Y (1984) Characterization of gamma-linolenic acid in Ribes seed. Lipids 19: 923-928.
• Tsevegsuren N, Aitzetmuller K (1996) Gamma linolenic and stearidonic acids in Mongolian Boraginaceae. J Am Oil Chem Soc 73: 1681-1684.
• Velasco L, Goffman FD (1999) Chemotaxonomic significance of fatty acids and tocopherols in Boraginaceae Phytochemistry 52: 423-426.
• Wolf RB, Kleiman R, England RE (1987) New sources of γ-linolenic acid. J Am Oil Chem Soc 60: 1858-1860.
• Zając A, Zając M (2001) Distribution Atlas of Vascular Plants in Poland, p 205. Institute of Botany, Jagiellonian University. Cracow