Analysis of individual azurocidin N-glycosylation sites in regard to its secretion by insect cells, susceptibility to proteolysis and antibacterial activity

• Authors: Katarzyna Indyk , Teresa Olczak , Justyna Ciuraszkiewicz , Wiesław Wątorek , Mariusz Olczak
• Languages of publication: EN

Abstracts

EN

Azurocidin is an inactive serine protease homolog with primary sequence similarity to neutrophil elastase, cathepsin G, and proteinase 3. The aim of this study was to investigate possible consequences of differential glycosylation of azurocidin in regard to its secretion, protein stability as measured by susceptibility to proteolysis, and antibacterial activity. Site-directed mutagenesis was employed to generate mutant azurocidin variants lacking individual N-glycosylation sites. Our results show that N-linked glycans may play a role in proper azurocidin folding and subsequent secretion by insect cells. We also demonstrate that N-linked glycosylation contributes to azurocidin stability by protecting it from proteolysis. The lack of N-glycosylation at individual sites does not significantly influence the azurocidin antibacterial activity.

Keywords

EN

glycosylation; protein secretion; azurocidin; antimicrobial activity

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 3
• Pages: 567-573

Dates

published

2007

received

2007-02-24

revised

2007-06-21

accepted

2007-07-06

(unknown)

2007-07-26

Contributors

author

Katarzyna Indyk

• Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Teresa Olczak

• Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Justyna Ciuraszkiewicz

• Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Wiesław Wątorek

• Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

author

Mariusz Olczak

• Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

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