Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells

• Authors: Kyung Hee Jung , Eunyoung Ha , Mi-Ja Kim , Yoon Kyung Uhm , Hye Kyung Kim , Seung-Jae Hong , Joo-Ho Chung , Sung-Vin Yim
• Languages of publication: EN

Abstracts

EN

The effect of Ganoderma lucidum extract on glucose uptake was studied in L6 rat skeletal muscle cells. G. lucidum extract increased glucose uptake about 2-fold compared to control. The extract stimulated the activity of phosphatidylinositol (PI) 3-kinase which is a major regulatory molecule in the glucose uptake pathway. About 7-fold increased activity of a PI 3-kinase was observed after treatment with G. lucidum extract, whereas PI 3-kinase inhibitor, LY294002, blocked the G. lucidum extract-stimulated PI 3-kinase activity in L6 skeletal muscle cells. Protein kinase B, a downstream mediator of PI 3-kinase, was also activated by G. lucidum extract. We then assessed the activity of AMP-activated protein kinase (AMPK), another regulatory molecule in the glucose uptake pathway. G. lucidum extract increased the phosphorylation level of both AMPK α1 and α2. Activity of p38 MAPK, a downstream mediator of AMPK, was also increased by G. lucidum extract. Taken together, these results suggest that G. lucidum extract may stimulate glucose uptake, through both PI 3-kinase and AMPK in L6 skeletal muscle cells thereby contributing to glucose homeostasis.

Keywords

EN

Ganoderma lucidum extract; skeletal muscle; phosphatidylinositol 3-kinase; AMP-activated protein kinase; glucose uptake

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 597-601

Dates

published

2006

received

2006-03-29

revised

2006-08-18

accepted

2006-09-08

(unknown)

2006-09-10

Contributors

author

Kyung Hee Jung

• Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

author

Eunyoung Ha

• Department of Biochemistry, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

author

Mi-Ja Kim

• Department of Obesity Science, Dongduk Women's University, Seoul, Republic of Korea

author

Yoon Kyung Uhm

• Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

author

Hye Kyung Kim

• Department of Food and Biotechnology, Hanseo University, Seosan, Republic of Korea, Seoul, Republic of Korea

author

Seung-Jae Hong

• Division of Rheumatology, Department of Internal Medicine, Kyung Hee Medical Center, Seoul, Republic of Korea

author

Joo-Ho Chung

• Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

author

Sung-Vin Yim

• Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

References

• Baron AD, Laakso M, Brechtel G, Edelman SV (1991) J Clin Invest 87: 1186-1194.
• Brazil D, Yang ZZ, Hemmings B (2004) Trends Biochem Sci 29: 233-242.
• Bjornholm M, Kawano Y, Lehtihet M, Zierath JR (1997) Diabetes 46: 524-527.
• Brozinick J Jr, Birnbaum MJ (1998) J Biol Chem 273: 14679-14682.
• Cao Y, Cao H, Chan E, Tang W, Xu A, Yang H, Huang M, Lan J, Li X, Duan W, Xu C, Zhou S (2005) Immunol Invest 34: 171-198.
• Ceddia RB, Somwar R, Maida A, Fang X, Bikopoulos G, Sweeney G (2005) Diabetologia 48: 132-139.
• Chan TO, Rittenhouse SE, Tsichlis PN (1999) Annu Rev Biochem 68: 965-1014.
• Cheatham B, Vlahos CJ, Cheatham L, Wang L, Blenis J, Kahn CR (1994) Mol Cell Biol 14: 4902-4911.
• Cheung PC, Salt IP, Davies SP, Hardie DG, Carling D (2000) Biochem J 346: 659-669.
• Choi YK, Kim TK, Kim CJ, Lee JS, Oh SY, Joo HS, Foster DN, Hong KC, You S, Kim H (2006) Exp Mol Med 38: 11-17.
• Cortright RN, Dohm GL (1997) Can J Appl Physiol 22: 519-530.
• Dohm GL, Tapscott EB, Pories WJ, Dabbs DJ, Flickinger EG, Meelheim D, Fushiki T, Atkinson SM, Elton CW, Caro JF (1988) J Clin Invest 82: 486-494.
• Eichhorn J, Kayali AG, Austin DA, Webster NJ (2001) Biochem Biophys Res Commun 282: 615-620.
• Franz G (1989) Planta Med 55: 493-497.
• Furusawa E, Chou SC, Furusawa S, Hirazami A, Dang Y (1992) Phytother Res 6: 300-304.
• Ha E, Yim SV, Jung KH, Yoon SH, Zheng LT, Kim MJ, Hong SJ, Choe BK, Baik HH, Chung JH, Kim JW (2006) Pharmacogenomics J 6: 327-332.
• Hayashi T, Hirshman MF, Kurth EJ, Winder WW, Goodyear LJ (1998) Diabetes 47: 1369-1373.
• Hikino H, Konno C, Mirin Y, Hayashi T (1985) Planta Med 4: 339-340.
• Kabir Y, Kimura S, Tamura T (1988) J Nutr Sci Vitaminol 34: 433-438.
• Kim DH, Shim SB, Kim NJ, Jang IS (1999) Biol Pharm Bull 22: 162-164.
• Kimura Y (2005) In Vivo 19: 37-60.
• Longnus SL, Segalen C, Giudicelli J, Sajan MP, Farese RV, Van Obberghen E (2005) Diabetologia 48: 2591-2601.
• Maleppillil VV, Sandeep S, Rishi RC (2005) Britis J Pharm 10: 1-8.
• Musi N, Goodyear LJ (2002) Curr Drug Targets Immune Endocr Metabol Disord 2: 119-127.
• Musi N, Hayashi T, Fujii N, Hirshman MF, Witters LA, Goodyear LJ (2001) Am J Physiol Endocrinol Metab 280: E677-E684.
• Mu J, Brozinick JT Jr, Valladares O, Bucan M, Birnbaum MJ (2001) Mol Cell 7: 1085-1094.
• Ouchi N, Kobayashi H, Kihara S, Kumada M, Sato K, Inoue T, Funahashi T, Walsh K (2004) J Biol Chem 279: 1304-1309.
• Salt IP, Connell JM, Gould GW (2000) Diabetes 49: 1649-1656.
• Stapleton D, Mitchelhill KI, Gao G, Widmer J, Mitchell BJ, Teh T, House CM, Fernandez CS, Cox T, Witters LA, Kemp BE (1996) J Biol Chem 271: 611-614.
• Thornton C, Snowden M A, Carling D (1998) J Biol Chem 273: 12443-12450.
• Vavvas D, Apazidis A, Saha AK, Gamble J, Patel A, Kemp BE, Witters LA, Ruderman NB (1997) J Biol Chem 272: 13255-13261.
• Wang SY, Hsu ML, Hsu HC, Tzeng CH, Lee SS, Shiao MS, Ho CK (1997) Int J Cancer 70: 699-705.
• Wasser SP, Weis AL (1999) Crit Rev Immunol 19: 65-96.
• Xi X, Han J, Zhang JZ (2001) J Biol Chem 276: 41029-41034.
• Zhang HN, Lin ZB (2004) Acta Pharmacol Sin 25: 191-195.
• Zierath JR, Krook A, Wallberg-Henriksson H (2000) Diabetologia 43: 821-835.
• Zou MH, Kirkpatrick SS, Davis BJ, Nelson JS, Wiles WG 4th, Schlattner U, Neumann D, Brownlee M, Freeman MB, Goldman MH (2004) J Biol Chem 279: 43940- 43945.