CD40 stimulation induces differentiation of acute lymphoblastic leukemia cells into dendritic cells

• Authors: Włodzimierz Łuczyński , Anna Stasiak-Barmuta , Elżbieta Iłendo , Maryna Krawczuk-Rybak , Iwona Malinowska , Małgorzata Mitura-Lesiuk , Adam Parfieńczyk , Marcin Szymański
• Languages of publication: EN

Abstracts

EN

Despite the very high percentage of long-term remissions in acute lymphoblastic leukemia (ALL) in children, some of them suffer from recurrence of the disease. New treatment modalities, e.g. effective geno- and immunotherapy are needed. The use of neoplasmatic cells to present tumor antigens is one of the approaches in cancer vaccines. ALL cells lack the expression of costimulatory molecules and are poor antigen presenting cells (APCs) for T-cell activation. CD40/40L interaction stimulates B-cells to proliferate, differentiate, upregulate costimulatory molecules and increase antigen presentation. The aim of the study was to test the hypothesis that ALL cells can be turned into professional APCs by CD40L activation. Children with B-cell precursor ALL were enrolled into the study. Mononuclear cells from bone marrow or peripheral blood were stimulated with CD40L and interleukin 4. Results: 1) after culture we noted upregulation of all assessed costimulatory, adhesion and activatory molecules i.e. CD1a, CD11c, CD40, CD54, CD80, CD83, CD86, CD123, HLA class I and II; 2) CD40L activated ALL cells induced proliferation of allogeneic T-cells (measured by [3H]thymidine incorporation). These results confirm the possibility of enhancing the immunogenicity of ALL cells with the CD40L system and indicate that this approach can be used in immunotherapeutic trials.

Keywords

EN

immunotherapy; T-lymphocytes; CD40L; acute lymphoblastic leukemia; dendritic cells

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 2
• Pages: 377-382

Dates

published

2006

received

2005-11-18

revised

2006-03-22

accepted

2006-04-19

(unknown)

2006-05-29

Contributors

author

Włodzimierz Łuczyński

• Department of Pediatric Oncology, Medical University of Białystok, Białystok, Poland

author

Anna Stasiak-Barmuta

• Department of Flow Cytometry, Medical University of Białystok, Białystok, Poland

author

Elżbieta Iłendo

• Department of Cytogenetics, Medical University of Białystok, Białystok, Poland

author

Maryna Krawczuk-Rybak

• Department of Pediatric Oncology, Medical University of Białystok, Białystok, Poland

author

Iwona Malinowska

• Department of Pediatrics, Hematology and Oncology, Medical University of Warsaw, Warsaw, Poland

author

Małgorzata Mitura-Lesiuk

• Department of Pediatric Hematology and Oncology, Medical University of Lublin, Lublin, Poland

author

Adam Parfieńczyk

• Department of Nuclear Medicine, Medical University of Białystok, Białystok, Poland

author

Marcin Szymański

• Students Scientific Section at the Department of Pediatric Oncology, Medical University of Białystok, Białystok, Poland

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