Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum

• Authors: Alina Krystynowicz , Maria Koziołkiewicz , Agnieszka Wiktorowska-Jezierska , Stanisław Bielecki , Emilia Klemenska , Aleksander Masny , Andrzej Płucienniczak
• Languages of publication: EN

Abstracts

EN

Acetobacter xylinum strains are known as very efficient producers of bacterial cellulose which, due to its unique properties, has great application potential. One of the most important problems faced during cellulose synthesis by these bacteria is generation of cellulose non-producing cells, which can appear under submerged culture conditions. The reasons of this remain unknow. These studies have been undertaken to compare at the molecular level wild-type, cellulose producing (Cel+) A. xylinum strains with Cel- forms of cellulose-negative phenotype. Comparison of protein profiles of both forms of A. xylinum by 2D electrophoresis allowed for the isolation of proteins which were produced exclusively by either Cel+ or Cel- cells. Sequences of peptides derived from these proteins were aligned with those of proteins deposited in databases. This analysis revealed that Cel- cells lacked two enzymes: phosphoglucomutase and glucose-1-phosphate uridylyltransferase, which generates UDP-glucose being the substrate for cellulose synthase. DNA was analyzed by ligation-mediated PCR carried out at low denaturation temperature (PCR-MP). Two DNA fragments of different thermal stability (218 and 217 bp) were obtained from the DNA of Cel+ and Cel- forms, respectively. The only difference between these Cel- and Cel+ DNA fragments is deletion of one T residue. Alignment of those two sequences with those deposited in the GenBank database revealed that similar fragments are present in the genomes of some bacterial cellulose producers and are located downstream from open reading frames (ORF) encoding phosphoglucomutase. The meaning of this observation is discussed.

Keywords

EN

2-D electrophoresis; UDP-glucose pyrophosphorylase; phosphoglucomutase; bacterial cellulose; Acetobacter xylinum; PCR-MP

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 3
• Pages: 691-698

Dates

published

2005

received

2005-07-13

revised

2005-07-25

accepted

2005-07-28

(unknown)

2005-09-01

Contributors

author

Alina Krystynowicz

• Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland

author

Maria Koziołkiewicz

• Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland

author

Agnieszka Wiktorowska-Jezierska

• Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland

author

Stanisław Bielecki

• Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland

author

Emilia Klemenska

• Institute of Biotechnology and Antibiotics, Warszawa, Poland

author

Aleksander Masny

• Institute of Biotechnology and Antibiotics, Warszawa, Poland

author

Andrzej Płucienniczak

• Institute of Biotechnology and Antibiotics, Warszawa, Poland

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