Fibronectin fragments in human seminal plasma.

• Authors: Iwona Kątnik-Prastowska , Magdalena Przybysz , Anna Chełmońska-Soyta
• Languages of publication: EN

Abstracts

EN

The study has revealed the presence of fibronectin (FN) fragments and a lack of intact FN in 72 seminal plasma samples. The FN fragmentation was examined by immunoblotting with a monoclonal antibody specific to the central cellular FN domain and was confirmed with a monoclonal antibody directed to the C-terminal domain of FN. Nine FN fragments between 60 and 200 kDa and five fragments of 60-150 kDa were identified in seminal plasma samples of normozoospermic and of terato-, oligoterato-, and oligoasthenoterato-spermic groups, respectively. The relative amounts of the 60, 90 and 100 kDa FN fragments were 2-3 times higher in seminal plasmas with abnormal semen characteristics than in the normozoospermic group. The results suggest that seminal plasma FN fragments may contribute to fertilization and the analysis of FN fragmentation may have a diagnostic value in andrological investigations.

Keywords

EN

cellular domain of fibronectin; seminal plasma; fibronectin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2005
• Volume: 52
• Issue: 2
• Pages: 557-560

Dates

published

2005

received

2004-05-13

revised

2004-11-22

accepted

2004-12-10

(unknown)

2005-05-15

Contributors

author

Iwona Kątnik-Prastowska

• Department of Chemistry and Immunochemistry, Medical University, Wrocław, Poland

author

Magdalena Przybysz

• Department of Chemistry and Immunochemistry, Medical University, Wrocław, Poland

author

Anna Chełmońska-Soyta

• Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

References

• Barilla ML, Carsons SE (2000) Semin Arthritis Rheum 29: 252-265.
• Fukai F, Ohtaki M, Fujii N, Yajima H, Ishii T, Nishizawa Y, Miyazaki K, Katayama T (1995) Biochemistry 34: 11453-11459.
• Grant MB, Caballero S, Bush DM, Spoerri PE (1998) Diabetes 47: 1335-1340.
• Hashino K, Uemori Y, Kimizuka F, Kato I, Titani K (1996) J Biochem 119: 604-609.
• Huynh QN, Wang S, Tafolla E, Gansky SA, Kapila S, Armitage GC, Kapila YL (2002) J Periodontol 73: 1101-1110.
• James K, Skibinski G (1995) In: Immunology of Human Reproduction. Kurpisz M, Fernandez N, eds, pp 267-283. BIOS Scientific Publishers Ltd.
• Labat-Robert J (2002) Semin Cancer Biol 12: 187-195.
• Laemmli UK (1970) Nature 227: 680-685.
• Lilja H, Oldbring J, Rannevik G, Laurell CB (1987) J Clin Invest 80: 281-285.
• Pinke LA, Swanlund AJ, Hensleigh HC, McCarthy JB, Roberts K, Pryor JL (1997) J Urol 158: 936-941.
• Romberger DJ (1997) Int J Biochem Cell Biol 29: 939-943.
• Stanton KJ, Frewin MB, Gudewicz PW (1999) J Leukocyte Biol 65: 515-522.
• Stauffer ChW, Parsons CL (1989) Urology 34: 80-85.
• Tchougounova E, Forsberg E, Angelborg G, Kjellen L, Pejler G (2001) J Biol Chem 276: 3772-3777.
• Wassarman P, Litscher ES (2001) Cells Tissues Organs 168: 36-45.
• Wennemuth G, Meinhardt A, Mallidis C, Albrecht M, Krause W, Renneberg H, Aumüller G (2000) Andrologia 33: 43-46.
• Yamada KM (2000) J Clin Invest 105: 1507-1550.
• Yi M, Ruoslahti E (2001) Proc Natl Acad Sci USA 98: 620-624.